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KMID : 0903519940370060447
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1994 Volume.37 No. 6 p.447 ~ p.450
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Hydrolysis of Phosphate Diesters as Nucleic Acid Model
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Abstract
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Rate of hydrolysis ethylene phosphate, dimethylphosphate and hydroxyethylmethylphosphate in neutral water have been measured. Hydrolysis of ethylene phosphate proceeds with P-0 bond cleavage (k_(obs)=3×10^(-7)s^(-1) at 100℃, △H^*=24 ㎉, △S^*=25.5 eu). In constrast, hydrolysis of dimethylphosphate proceeds with C-O bond cleavage (k_(obs)=3×10^(-11)s^(-1) at 150℃). The rate constant for P-O bond cleavage of dimethylphosphate is estimated at 1×10^(-11)s^(-1) at 150℃ (△H^*=36 ㎉, △S^*=25.5 eu). A phosphodiesterase catalyzed hydrolysis of dimethylphosphate is 10^(17) times faster than the simple water rate. The observed rate of hydrolysis of hydroxyethylmethylphosphate is comparable to that of dimethylphosphate indicating C-O bond cleavage (k_(obs)=6×10^(-7)s^(-1) at 150℃).
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